Extended Data Fig. 1: Graphical User Interface of ANPELA and Preparation of Required Data. | Nature Protocols

Extended Data Fig. 1: Graphical User Interface of ANPELA and Preparation of Required Data.

From: Navigating the data processing for cytometry-based single-cell proteomics

Extended Data Fig. 1: Graphical User Interface of ANPELA and Preparation of Required Data.The alternative text for this image may have been generated using AI.

(a) The navigation bar of ANPELA software included ‘HOME’, ‘Single-cell Proteomics’, ‘Textual Tutorial’, and ‘Interactive Tutorial’. Clicking on ‘Single-cell Proteomics’ initiated data upload and processing. Clicking on ‘Textual Tutorial’ permitted downloading the textual tutorial. Clicking on ‘Interactive Tutorial’ opened a step-by-step interactive tutorial. (b) The essential data required by ANPELA included FCS files (i.e., raw data files) generated from cytometry-based SCP experiments and a metadata file describing the correlation between the raw data and experimental conditions. The metadata file was a user-created file named ‘metadata.csv’, containing key information in two columns.

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