Table 1 The identification of m⁵C sites in all 38 samples.

From: Transcriptome-wide RNA 5-methylcytosine profiles of human iPSCs and iPSC-derived cardiomyocytes

Reads mapped to:		Human reference genome Hg38				Spike-in control: Unmethylated firefly-luciferase mRNA
Sequenced sample libraries	Pooled Filtered reads	Mapped reads	% reads mapped /filtered	#m⁵C; mcov = 10 mr0.05 FDR = 0.05	C- > T conversion rate	Mapped Reads	% reads mapped /filtered	#m⁵C; mcov = 10 mr = 0.05 FDR = 0.01	C- > T conversion rate
hiPSCs(1103)-1	22,074,598	17,704,062	80.20%	51,329	0.9987	1,367	0.01%	0	0.9987
hiPSCs(1103)-2	17,034,687	13,339,820	78.31%	44,401	0.9989	850	0.00%	0	0.9986
hiPSCs(1103)-3	16,850,669	13,031,555	77.34%	38,985	0.9991	1,073	0.01%	3	0.9989
hiPSC(1103)-CM-1	17,400,153	13,492,390	77.54%	64,260	0.9988	2,021	0.01%	17	0.9969
hiPSC(1103)-CM-2	34,755,661	25,044,988	72.06%	89,574	0.9988	13,905	0.04%	0	0.9986
hiPSC(1103)-CM-3	9,004,846	6,259,766	69.52%	35,157	0.9981	3,120	0.03%	2	0.9988
hiPSCs(1103)-CM-lac-1	39,186,930	31,443,665	80.24%	86,454	0.9987	4,014	0.010%	6	0.9984
hiPSCs(1103)-CM-lac-2	41,428,004	33,076,311	79.84%	118,053	0.9976	3,928	0.009%	3	0.9983
hiPSCs(1103)-CM-lac-3	47,536,604	37,793,708	79.50%	118,414	0.9983	4,254	0.009%	2	0.9982
hiPSCs(1103)-CM-TDI-1	44,057,030	36,074,397	81.88%	109,258	0.9985	2,750	0.006%	1	0.9986
hiPSCs(1103)-CM-TDI-2	46,927,187	38,099,690	81.19%	111,406	0.9985	3,074	0.007%	1	0.9988
hiPSCs(1103)-CM-TDI-3	42,097,493	33,361,703	79.25%	107,583	0.9982	3,840	0.009%	9	0.998
hAFSC-iPSC-1	58,925,084	43,048,724	73.06%	50,259	0.9992	2,604	0.00%	3	0.9988
hAFSC-iPSC-2	19,915,577	14,043,081	70.51%	51,566	0.9986	1,630,158	8.19%	23	0.9986
hAFSC-iPSC-3	54,956,886	41,816,608	76.09%	117,759	0.9984	3,223	0.01%	2	0.9989
hAFSC-iPSC-4	46,871,023	34,968,133	74.61%	120,350	0.9988	2,893	0.01%	12	0.9966
hAFSC-iPSC-CM-1	53,679,189	37,031,345	68.99%	76,453	0.989	2,702	0.01%	1	0.9987
hAFSC-iPSC-CM-2	37,217,977	27,178,049	73.02%	98,359	0.9992	3,498	0.01%	2	0.9979
hAFSC-iPSC-CM-3	41,695,404	27,531,206	66.03%	179,246	0.9987	3,332	0.01%	1	0.9988
hAFSC-iPSC-CM-lac-1	40,589,291	30,804,171	75.89%	99,608	0.998	1,908	0.005%	2	0.9988
hAFSC-iPSC-CM-lac-2	41,160,545	31,899,272	77.50%	110,118	0.9978	1,700	0.004%	2	0.9984
hAFSC-iPSC-CM-lac-3	45,481,881	34,872,648	76.67%	98,658	0.998	1,820	0.004%	2	0.9984
hAFSC-iPSC-CM-TDI-1	42,788,928	32,733,167	76.50%	93,232	0.9983	1,800	0.004%	4	0.9984
hAFSC-iPSC-CM-TDI-2	37,416,855	28,696,409	76.69%	88,459	0.9982	1,762	0.005%	2	0.998
hAFSC-iPSC-CM-TDI-3	32,172,657	24,567,063	76.36%	86,031	0.9979	1,226	0.004%	3	0.9989
hESC(RUES2)-1	55,636,450	40,518,423	72.83%	56,766	0.9991	2,013	0.00%	1	0.9987
hESC(RUES2)-2	17,624,476	12,754,417	72.37%	51,950	0.9988	1,452,791	8.24%	2	0.9986
hESC(RUES2)-3	46,009,412	34,978,457	76.02%	270,190	0.9964	1,656	0.00%	1	0.999
hESC(RUES2)-4	49,007,850	36,482,544	74.44%	107,529	0.9987	2,140	0.00%	1	0.9992
hESC-CM(RUES2-CM)-1	54,823,858	39,558,387	72.16%	56,971	0.9991	2,208	0.00%	0	0.999
hESC-CM(RUES2-CM)-2	20,146,987	14,896,408	73.94%	28,886	0.9994	680	0.00%	3	0.9985
hESC-CM(RUES2-CM)-3	35,517,244	26,964,049	75.92%	59,842	0.9994	950	0.00%	2	0.9991
hESC(RUES2)-CM-lac -1	52,093,456	40,635,437	78.00%	164,763	0.9978	2,708	0.005%	4	0.9978
hESC(RUES2)-CM-lac -2	51,582,269	39,601,331	76.77%	163,947	0.9979	3,442	0.007%	8	0.9982
hESC(RUES2)-CM-lac -3	52,628,240	40,606,338	77.16%	183,351	0.9973	2,594	0.005%	5	0.9988
hESC(RUES2)-CM-TDI -1	40,932,345	32,232,377	78.75%	127,776	0.9976	2,152	0.005%	2	0.9988
hESC(RUES2)-CM-TDI -2	38,049,403	30,194,759	79.36%	99,369	0.9983	2,596	0.007%	4	0.9984
hESC(RUES2)-CM-TDI -3	39,945,971	31,330,641	78.43%	113,880	0.9979	1,980	0.005%	6	0.9977

Table 1. BT-mRNA seq libraries were mapped to the in silico-converted reference genome and sequence. #m⁵C identification includes three criteria: (1) minimum coverage of 10 reads, (2) minimum methylation ratio 0.05, and (3) false discovery rate of 0.05 in reference genome or 0.01 in spike-in sequence.

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Table 1 The identification of m⁵C sites in all 38 samples.

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