Table 1 Experimental Conditions. This table details the channel specifications and experimental settings for each movie entry in the dataset. Cell types visible in each channel include neutrophils (Neu), dendritic cells (DCs), and eosinophils (Eos). Additionally, when applicable, the channels include second harmonic generation (SHG) and vaccine visualization. Experimental settings cover the used cell markers, fluorophores, target organ, applied stimuli, and microscope magnification.
From: An in vivo microscopy dataset for the characterization of leukocyte death
Video ID | CH1 | CH2 | CH3 | CH4 | Markers | Fluorophore | Organ | Stimuli | Objective |
|---|---|---|---|---|---|---|---|---|---|
Den1 | Den | Melanoma | Melanoma | — | CD11c, B16 | EYFP, mCherry | Lymph node | melanoma | 40x |
Den2 | Den | WT-Eos | KO Eos | — | not known, CK6, not specified | GFP, CMTMR, CFP | Spleen | UVPR8 | 40x |
Den3 | Den | Neu | SHG | — | CXCR3, Ly6G | GFP, CMTMR | Lymph node | UVPR8 | 40x |
Den4 | Den | NK | — | — | CD11c, NK1.1/NKp46 | YFP, CMTMR | Lymph node | UVPR8 | 40x |
Eos1 | Den | WT Eos | KO Eos | — | CX3CR1, IL—5, IL-5 | GFP, CMTMR, CTV | Spleen | UVPR8 | 16x |
Eos2 | Den | WT Eos | KO Eos | — | CX3CR1, IL-5, IL-5 | GFP, CMTMR, CTV | Spleen | UVPR8 | 40x |
Eos3 | Den | WT Eos | KO Eos | — | CX3CR1, IL-5, IL-5 | GFP, CMTMR, CTV | Spleen | UVPR8 | 40x |
Eos4 | Den | WT Eos | KO Eos | — | CX3CR1, IL-5, IL-5 | GFP, CMTMR, CTV | Spleen | UVPR8 | 40x |
Eos5 | Den | WT Eos | KO Eos | — | CX3CR1, IL-5, IL-5 | GFP, CMTMR, CTV | Spleen | UVPR8 | 40x |
Neu1 | Neu, NK | UVPR8 | Neu, (follicle) | — | CK6, Ncr1, CD21/35 | CFP, GFP, DiD, PB | Lymph node | UVPR8 | 20x |
Neu2 | SHG | UVPR8 | — | Neu | LysM-Cre | CFP | Lymph node | UVPR8 | 40x |
Neu3 | Neu | Macro | UVPR8 | — | LysM-Cre, CD169, UVPR8 | GFP, PE, DiD | Lymph node | UVPR8 | 10x |
Neu4 | Neu | UVPR8 | Neu, SHG | — | CK6 | CFP | Lymph node | UVPR8 | 16x |
Neu5 | Neu | UVPR8 | Neu, SHG | — | CK6 | CFP | Lymph node | UVPR8 | 16x |
Neu6 | Neu | — | SHG | — | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu7 | Naïve CD8 + T cell | Neu | Cognate CD8 + T cell | — | cell membrane, UBC | CMTMR, GFP, CTV | Spleen | None | 16x |
Neu 8 | Neu | Macro | — | Neu, SHG | (reporter), CD169, | CFP, PE | Lymph node | UVPR8 | 40x |
Neu 9 | Neu | UVPR8 | — | — | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu 10 | Neu, Neu | UVPR8 | Neu, SHG | — | CK6, UBC | CFP, GFP | Lymph node | UVPR8 | 16x |
Neu11 | Naïve CD8 + T cell | Neu | Cognate CD8 + T cell | — | cell membrane, UBC | CMTMR, GFP, CTV | Spleen | None | 16x |
Neu 12 | Neu, DC | Neu | Neu | — | (reporter), CD11c, P2X7, | CFP, YFP, CMTMR | Lymph node | UVPR8 | 16x |
Neu 13 | FDC | — | — | Neu | 8C12, (reporter) | Alexa fluor 568,CFP | Lymph node | UVPR8 | 20x |
Neu14 | Neu | UVPR8 | SHG | — | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu15 | Neu | UVPR8 | SHG | — | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu16 | Neu | — | SHG | UVPR9 | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu 17 | Neu | UVPR8 | Neu | SHG | UBC, (cell membrane) | EGFP, Cell trace Far Red | Spleen | UVPR8 | 40x |
Neu18 | Neu | UVPR8 | SHG | — | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu 19 | Neu,DC | UVPR8 | Neu, SHG | — | CK6, CD11c | CFP, YFP | Lymph node | UVPR8 | 40x |
Neu 20 | Dc | UVPR8 | — | Neu | CD11c, (reporter) | YFP, CFP | Lymph node | UPPR8 | 40x |
Neu 21 | Neu | UVPR8 | — | — | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu 22 | Neu | UVPR8 | — | — | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu 23 | Neu | UVPR8 | — | — | UBC | EGFP | Spleen | UVPR8 | 16x |
Neu 24 | Neu,DC | UVPR8 | Neu, SHG | — | CK6, CD11c | CFP, YFP | Spleen | UVPR8 | 16x |
Neu25 | Neu | Den | — | SHG | CD11c, Ly6G | CMTMR, YFP | Lymph node | UVPR8 | 40x |
