Fig. 2

Sequencing Quality Assessment. (a) Distribution of full-length sequences after redundancy removal. The x-axis denotes the read length, while the y-axis represents the number of reads within the corresponding length range. The dashed line indicates the N50 length.(b) Comparative analysis of transcript identification results between all detected transcripts and reference transcripts. The red bars indicate the number of identified genes, whereas the blue bars denote the number of identified transcripts. (c) Heatmap illustrating Pearson correlation coefficients between samples based on all detected transcripts. Both the x-axis and y-axis represent individual samples, and the color intensity reflects the magnitude of correlation between pairs of samples. A redder color signifies stronger correlation. A correlation coefficient approaching 1 indicates greater similarity in expression patterns between samples.