Fig. 2

Data distribution and differential expression of Fmr1 gene in E14.5 telencephalon of Fmr1-mutant mouse embryos. MA plots illustrating overall data distribution by comparing male WT vs Fmr1-KO, female WT vs Fmr1-het, and female WT vs Fmr1-KO. Transcripts with significant differential expression (p < 0.05) are highlighted using colored datapoints, and Fmr1 gene symbol is indicated for each comparison. The Y-axis represents log₂ fold change, while the X-axis indicates the log₂ of the mean of normalized gene expression levels (TPM + 1) between groups (see Methods for more details). (a) Comparison of male WT (dark blue) vs male Fmr1-KO (light blue). (b) Comparison of female WT (red) vs female Fmr1-het (pink). (c) Comparison of female WT (red) vs female Fmr1-KO (light pink).