Table 4 Summary of de novo assembly and annotation statistics for transcriptomes from six Hua species.
From: De novo assembly of transcriptomes of six Hua species (Semisulcospiridae, Cerithioidea, Gastropoda)
Metrics | Hua textrix (SEM-A1) | Hua yangi (SEM-B1) | Hua sp. 1 (SEM-C1) | Hua sp. 2 (SEM-D1) | Hua sp. 3 (GZA-001A) | Hua wujiangensis (GZB-001A) |
|---|---|---|---|---|---|---|
Raw Reads (M) | 43.4 | 47.2 | 40.6 | 39.5 | 55.9 | 62.0 |
Cleaned Reads (M) | 41.3 | 45.1 | 38.9 | 36.7 | 53.4 | 59.3 |
Transcripts | 147,147 | 166,059 | 176,784 | 158,952 | 252,938 | 268,741 |
Trinity “genes” | 112,211 | 120,699 | 125,890 | 120,980 | 175,267 | 173,907 |
Median length (bp) | 514 | 502 | 440 | 484 | 399 | 437 |
Average length (bp) | 866.8 | 883.3 | 723.9 | 817.5 | 716.6 | 797.0 |
GC (%) | 46.31 | 46.41 | 45.95 | 46.08 | 46.93 | 46.82 |
Contig N50 | 1,306 | 1,386 | 1,010 | 979 | 1,086 | 1,250 |
Complete BUSCOs | 3,950 (74.6%) | 4,019 (75.9%) | 3,129 (59.1%) | 3,637 (68.6%) | 3,930 (74.2%) | 4,447 (84.0%) |
Fragmented BUSCOs | 214 (4.0%) | 232 (4.4%) | 352 (6.6%) | 271 (5.1%) | 241 (4.6%) | 162 (3.1%) |
Missing BUSCOs | 1,131 (21.4%) | 1,044 (19.7%) | 1,814 (34.3%) | 1,387 (26.3%) | 1,124 (21.2%) | 686 (12.9%) |
Transcripts with BLAST hits | 18,069 | 18,926 | 20,327 | 18,281 | 29,884 | 24,433 |
Transcripts with GO terms | 17,649 | 18,503 | 19,889 | 17,884 | 29,374 | 23,980 |
Transcripts with KEGG pathways | 15,354 | 16,134 | 17,405 | 15,594 | 26,122 | 20,893 |
Transcripts with Pfam accession | 8,142 | 8,724 | 8,867 | 8,200 | 10,591 | 10,753 |
Transcripts with COG functional categories | 147 | 140 | 234 | 153 | 212 | 169 |
