Table 5 qPCR validation of select transcripts in rat lung.
From: Acute Changes in Rat Tissue Gene Expression Following Exposure to Flight Relevant Hypobaria
Gene | Biological group | Microarray results (fold change from baseline) | qPCR results (fold change from baseline) |
|---|---|---|---|
Hif3a | 100% O2, 0 ft, 10 hr | 1.622 | 2.6 |
Osgin1 | 100% O2, 0 ft, 10 hr | 1.534 | 2.096 |
Sod3 | 21% O2, 8000 ft, 10 hr | 1.669 | 1.09 |
Ntrk2 | 21% O2, 4000 ft, 5 hr | 2.604 | 2.899 |
Ntrk2 | 21% O2, 4000 ft, 10 hr | 2.879 | 3.67 |
Ntrk2 | 21% O2, 8000 ft, 5 hr | 11.875 | 14.572 |
Ntrk2 | 21% O2, 8000 ft, 10 hr | 12.122 | 16.607 |
Ntrk2 | 100% O2, 0 ft, 10 hr | −2.458 | −4.31 |
Ntrk2 | 100%, 4000 ft, 10 hr | −1.64 | −2.985 |
Zbtb16 | 21% O2, 8000 ft, 5 hr | 1.667 | −0.9 |
Zbtb16 | 21% O2, 8000 ft, 10 hr | 2.32 | 2.6 |
Zbtb16 | 100% O2, 0 ft, 10 hr | 2.218 | 1.9 |
Zbtb16 | 100% O2, 4000 ft, 10 hr | 2.144 | 1.645 |
Ttpa | 21% O2, 4000 ft, 5 hr | −1.605 | −1.908 |
Ttpa | 21% O2, 4000 ft, 10 hr | −1.799 | −1.27 |
Ttpa | 21% O2, 8000 ft, 5 hr | −1.624 | −2.445 |
Ttpa | 21% O2, 8000 ft 10 hr | −1.527 | −2.041 |
Fech | 100% O2, 4000 ft, 10 hr | −1.607 | −2.012 |
Npr3 | 21% O2, 8000 ft, 5 hr | −2.089 | −3.663 |
Npr3 | 21% O2, 8000 ft, 10 hr | −2.39 | −5 |
Slc4a1 | 21% O2, 4000 ft, 5 hr | −1.78 | −2.577 |
Slc4a1 | 21% O2, 8000 ft, 10 hr | −1.576 | −2.353 |
Slc4a1 | 100% O2, 0 ft, 5 hr | −1.597 | −1.718 |
Slc4a1 | 100% O2, 0 ft, 10 hr | −1.885 | −2.237 |
Slc4a1 | 100% O2, 4000 ft, 5 hr | −2.038 | −1.802 |
Slc4a1 | 100% O2, 4000 ft, 10 hr | −2.145 | −2.681 |
Alas2 | 100% O2, 0 ft, 10 hr | −2.252 | −1.543 |
Alas2 | 100% O2, 4000 ft, 5 hr | −2.01 | −1.429 |
Alas2 | 100% O2, 4000 ft, 10 hr | −2.091 | −1.667 |
