Fig. 1

Characterization of lncRNAs, circRNAs, and sncRNAsexpression profiles in BRAF^V600E-mutant CM cells and their Spry^KO clones. (a) Schematic representation of the experimental workflow followed to generate and validate the sncRNA datasets, including cross-analysis with lncRNAs and circRNAs from both in vitro and in vivo total RNA-seq experiments. (b) Bar plot showing the number of expressed lncRNAs and circRNAs in the indicated cell lines. Log₂-transformed values represent the average of replicates. (c) Bar plot showing the number of expressed miRNAs, novel miRNAs (nov-miRNAs), piRNAs, snRNAs, snoRNAs, and tRNAs in the indicated cell lines. Values represent an average of three replicates. (d) Circos plot showing genomic distribution of the first 1000 circRNAs, lncRNAs, and miRNAs detected (reads ≥ 10 for lncRNAs and circRNAs, reads ≥ 3 for miRNAs) in parental cell lines (left) and Spry1^KO clones (right).