Fig. 2

Validations of the experimental procedure. (a) Representative western blot and relative densitometry analysis of Spry1 protein expression in parental BRAF^V600E-mutant CM cell lines and their respective Spry1^KO clones. β-tubulin was used as a loading control. Images were processed with ImageJ software (https://imagej.Net) for densitometry readings. (b) Gel-like images of tape station analysis of total RNA samples from parental and Spry1^KO cell lines, showing RIN values. One representative replicate of three is shown for each cell line. EL indicates the electronic ladder. PCA of sncRNAs (c) and ncRNAs (d) in parental and Spry1^KO cells. Different shapes (circles, triangles, and squares) indicate biological/technical replicates, color-coded according to cell lines. The first two components (PC1 and PC2) are shown. (e) Correlation heatmaps of miRNAs (left), lncRNAs (middle), and circRNAs (right) expression across the samples.