Fig. 3
From: Light sheet microscopy imaging dataset of CAR-T-cell-mediated cytotoxicity
Data structure. (a) Schematic overview of the reconstructed data directory organization and file structure. (b) MIP of the 488 nm and 561 nm channels at a single time point, alongside their merged composite image. (c) Visualization of a 4D volumetric dataset (x, y, z, channel) for a single time point. (d) Schematic of the 3D U-Net segmentation masks, identifying distinct cellular compartments. (e) Merged imaging data of four-color labeled samples. The green channel represents CAR-T cell actin (EGFP). The red channel shows Nalm6 cells (mApple-Mem plasmid transfection) for the first five volumes, and tubulin (SiR-tubulin) for the sixth volume. The blue channel indicates the nuclei of dead cells, stained with Sytox blue dye upon cell death. (f) Single-channel imaging data.
