Figure 4 | Scientific Reports

Figure 4

From: Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins

Figure 4

C. difficile flagella-induced degradation of IκB-α. Caecal lysates were prepared as indicated in Material and Methods section and proteins were resolved by SDS-PAGE. (A) R20291 derivative-infected C57BL/6 mice; (B) 630Δerm derivative-infected C57BL/6 mice, and (C) R20291 derivative- or 630Δerm WT-infected C57BL/6 tlr5 −/− KO mice. Western blot were then performed using anti-IκB-α, actin antibodies. Western blot cropped pictures (full-length blots are in the Supplementary Information file) show the results of a representative experiment. The density of the bands was measured using Fusion software. Ratios IκB-α/actin were calculated and for the negative control (C-, uninfected mice), this ratio was normalized to 1. The ratio of the other samples was reported to the negative control. Results represent the mean (n = 10) ± standard deviations for each group of animals. *Statistically significant differences (P < 0.05) compared to the negative control.

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