Figure 1
From: Long intergenic non-coding RNA GALMD3 in chicken Marek’s disease
Identification and validation of linc-GALMD3 and its neighboring genes. (A) Validation of linc-GALMD3 expression. The mean value of log2 FC (Infected/Non-infected) was compared in the bar chart for CD4+ T cells from two reciprocal cross lines (63 × 72 and 72 × 63), respectively; FC means fold change; and the qRT-PCR data was normalized by GAPDH expression. Blue and red bars depict the RNA-Seq and qRT-PCR results, respectively. (B) Identification and conservation of linc-GALMD3 among species. The black bar represented linc-GALMD3, NSGALT00000007468 and NSGALT00000007459 were the transcripts of upstream and downstream coding genes of linc-GALMD3, and NSGALT000000290030 was the transcript of the downstream gga-miR-223 gene. (C) Expression of linc-GALMD3 neighboring genes in CD4+ T cells. Inf. means infected CD4+ T cells; non-inf. means non-infected CD4+ T cells; up/downstream gene expression were both normalized by GAPDH expression, ggg-miR-223 expression was normalized by chicken 5s rRNA expression, all the expression were normalized by the corresponding non-infection group; N = 3; and **p value < 0.01.
