Figure 3 | Scientific Reports

Figure 3

From: Synthesis, high-throughput screening and pharmacological characterization of β–lactam derivatives as TRPM8 antagonists

Figure 3

Compounds 41 and 45 strongly inhibited TRPM8-mediated currents. Left: Representative whole-cell voltage clamp recordings from TRPM8-expressing HEK-CR1 cells and compound 41 (A) and 45 (B). Voltage was held at −60 mV. Pre-application of compounds (20 s) was followed by co-application with 500 µM menthol (Control) for 20s. Current traces of different colours denote the different concentrations of compounds tested. Right: Dose response of compound 41 (A, red), 45 (B, blue) TRPM8 blocked activity. Solid line depicts the fitting to a Hill equation. Data are given as mean ± SEM, with n > 4 cells per data point.

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