Figure 1

Development of ‘Baoding’ alfalfa microspores in culture. (A) Flower buds at different developmental stages. (B) Swollen microspores after cell division, the arrows in B indicate these are swollen microspores. (C) Embryos of various sizes after 21 days of culture. (D) Callus formation stage. (E) Plantlets on B5 medium. (F) Uninucleate microspores, the arrows in F indicate these are uninucleate microspores.