Table 4 Effect of low temperature (4 °C) on alfalfa microspore embryogenesis in culture.

From: RETRACTED ARTICLE: Doubled haploid production in alfalfa (Medicago sativa L.) through isolated microspore culture

low-temperature treatments

Number of plantlets produced

Plantlets induction frequency

4 °C for 0 h

12

10.0% a

4 °C for 3 h

16

13.33% b

4 °C for 6 h

18

15.0% b

4 °C for 12 h

17

14.17% b

4 °C for 24 h

24

20.0% c

4 °C for 48 h

11

9.17% a

  1. Different letters following survival rate indicate statistically significant differences based on the Duncan’s new multiple range test at P < 0.05.
Back to article page