Table 5 Effect of heat shock (32 °C) on alfalfa microspore embryogenesis in culture.
low-temperature treatments | Number of plantlets produced | Plantlets induction frequency |
|---|---|---|
32 °C for 0 d | 9 | 7.5% a |
32 °C for 1 d | 12 | 10.0% b |
32 °C for 2 d | 17 | 14.17% c |
32 °C for 3 d | 13 | 10.83% b |
32 °C for 4 d | 9 | 7.5% a |
32 °C for 5 d | 1 | 0.83% d |
32 °C for 6 d | 0 | 0 e |
