Correction to: Scientific Reports https://doi.org/10.1038/s41598-018-30786-y, published online 17 August 2018
In Figure 5b, “EV Spike glucuronidase (UV)” should read “EV spike glucuronidase (90° LS)”. The correct Figure 5b appears below as Figure. 1.
Analysis of glucuronidase-loaded EVs by asymmetric flow field-flow fractionation (AF4). (a) The working principle of AF4 consists of an injection step with a simultaneous sample focussing. Elution from the flow channel combined with a tangential cross-flow allows separation of particles and compounds by size. (b) Representative chromatograms of injections of free enzyme (glucuronidase 0.5 mg/mL), unmodified EVs, EVs spiked with glucuronidase (0.05 mg/mL), and EV-glucuronidase loaded samples (freshly purified by SEC). Detection of glucuronidase and EVs was conducted by UV spectroscopy and light scattering at 90° (90° LS), respectively. Smaller enzyme molecules are eluting earlier (~15 min) and larger EVs later (~22 min). Free glucuronidase cannot be LS-detected due to low scattering intensity. (c) Glucuronidase-loaded EVs isolated from HUVEC cells were stored for 7 days at 4 °C, −80 °C and lyophilised with 4% (w/v) trehalose. Their enzymatic activity was assessed after purification by AF4 and normalised to the average activity before storage. For EVs analysis, only the peak centre (i.e., 21–22 min) was collected by AF4 and enzyme activity was measured using fluorescein β-D-glucuronide. Mean ± SD, n = 3.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Frank, J., Richter, M., de Rossi, C. et al. Author Correction: Extracellular vesicles protect glucuronidase model enzymes during freeze-drying. Sci Rep 9, 15702 (2019). https://doi.org/10.1038/s41598-019-48221-1
Published:
Version of record:
DOI: https://doi.org/10.1038/s41598-019-48221-1
