Table 2 Chemical composition of the extract of H. perforatum calli obtained from in vitro grown plants after treatment with perlite NPs and TiO2/perlite NCs.
>Compunds | Peak area % | Classification | RT | RI | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Control | P 25 (mg/L) | P 50 (mg/L) | P 100 (mg/L) | P 150 (mg/L) | P 200 (mg/L) | P + T 25 (mg/L) | P + T 50 (mg/L) | P + T 100 (mg/L) | P + T 150 (mg/L) | P + T 200 (mg/L) | ||||
Decane | — | — | — | 2.16 | — | — | — | — | — | — | — | Hydrocarbon-alkan | 7.28 | 962 |
4,7-Methano-1H-indene, 3a,4,7,7a-tetrahydro- | — | — | — | 2.09 | — | — | — | — | — | — | — | Hydrocarbon | 7.88 | 994 |
Undecane | — | — | — | 1.57 | — | — | — | — | — | — | — | Hydrocarbon-alkan | 9.31 | 1075 |
1. 3-Cyclohexene-1-acetaldehyde | 3.39 | — | — | — | — | — | — | — | — | — | — | Aldehyde | 9.32 | 1076 |
(1RS,2RS,4RS)−2-Methylbicyclo[2.2.2]oct-5-en-2-ol | — | — | — | 1.09 | — | — | — | — | — | — | — | Alcohol | 9.39 | 1077 |
Dodecane | — | — | — | 3.21 | — | — | — | — | — | — | — | Hydrocarbon-alkan | 11.2 | 1180 |
Tridecane | — | — | — | 4.53 | 4.45 | — | — | — | — | — | — | Hydrocarbon-alkan | 13 | 1258 |
Tetradecane | — | — | — | — | — | 6.48 | — | — | — | — | — | Hydrocarbon-alkan | 15.3 | 1360 |
Tridecane, 3-methyl- | — | — | — | 1 | — | — | — | — | — | — | — | Hydrocarbon-alkan | 15.31 | 1361 |
Farnesan | — | — | — | 1.9 | — | — | — | — | — | — | — | Sesquiterpen | 15.43 | 1367 |
Pentadecane | — | — | — | 7.55 | — | 12.24 | — | — | — | — | — | Hydrocarbon-alkan | 17.91 | 1479 |
Hexadecane | — | — | — | 8.67 | — | 12.26 | — | — | — | — | — | Hydrocarbon-alkan | 20.6 | 1584 |
Heptadecane | — | — | — | 8.11 | — | 14 | — | — | — | — | — | Hydrocarbon-alkan | 23 | 1689 |
Heptadecane, 3-methyl | — | — | — | 1.06 | — | — | — | — | — | — | — | Hydrocarbon-alkan | 24.39 | 1761 |
Carbamodithioic acid, dibutyl | — | — | — | — | 4.2 | — | — | — | — | — | — | Acid | 24.43 | 1763 |
Octadecane | — | — | — | 7.82 | — | 10.77 | — | — | — | — | — | Hydrocarbon | 24.9 | 1788 |
Hexadecane, 2,6,10,14-tetramethy | — | — | — | 7.2 | — | — | — | — | — | — | — | Diterpen | 25.1 | 1798 |
Nonadecane | — | — | — | 7.38 | — | 10.73 | — | — | — | — | — | Hydrocarbon-alkan | 26.6 | 1887 |
Hexadecanoic acid, methyl ester | — | — | — | 4.41 | — | 4.99 | — | — | — | — | — | Fatty acid | 27.2 | 1925 |
3,11-diheptyloxybenzo[c]benzo[a]phenanthrene | — | — | 10.48 | — | — | — | 4.12 | — | — | — | — | phenolic | 27.8 | 1963 |
Eicosane | — | — | — | 7.02 | — | 7.3 | — | — | — | — | — | Hydrocarbon-alkan | 28.22 | 1991 |
Heneicosane | — | — | — | 7.52 | — | 6.04 | — | — | — | — | — | Hydrocarbon-alkan | 29.7 | 2090 |
9-Octadecenoic acid, (E).Oleic acid | — | — | — | — | — | 7.2 | — | — | — | — | — | Fatty acid | 29.82 | 2098 |
6-Aza-5,7,12,14-tetrathiapentacene | 3.39 | — | — | — | — | — | — | — | — | — | — | Alkaloid | 30.22 | 2121 |
Docosane | — | — | — | 3.52 | — | — | — | — | — | — | — | Hydrocarbon-alkan | 31.44 | 2190 |
Tricosane | — | — | — | 2.3 | — | — | — | — | — | — | — | Hydrocarbon-alkan | 33.41 | 2292 |
Octadecane, 3-ethyl-5-(2-ethylbutyl)- | — | — | — | — | — | — | — | — | — | — | 1.43 | Hydrocarbon-alkan | 35.2 | 2396 |
1,3,6,9b-Tetraazaphenalene-4-carbonitrile, 7,9-dibromo-2-(dibromomethyl)- | — | — | — | — | — | — | — | — | — | — | 3.72 | Alkaloid | 36.38 | 2474 |
Pentacosane | — | — | — | — | — | — | — | — | — | 7.91 | — | Hydrocarbon-alkan | 36.8 | 2502 |
1H-Indole, 2-methyl-3-phenyl- | — | — | — | — | — | — | — | — | — | — | 2.58 | Alkaloid-Indole | 37.24 | 2534 |
N-METHYLDEACETYLCOLCHICINE | — | — | 0.95 | — | — | — | — | — | — | — | — | Alkaloid | 37.47 | 2551 |
(Z,Z)-4-Ethyl-3-methyl-5-(5-4-aminophenyl-2-methylen)-3,4-dimethyl-5H-pyrrolyl-2-methylene)-3-pyrrolin-2-on | — | — | — | — | — | — | 4.94 | — | — | — | — | Alkaloid | 37.7 | 2568 |
2-[4-Cyclohexylbutanoylamino]-3-chloro-1,4-naphthoquinone | — | 1.48 | — | — | — | — | — | 3.17 | — | — | — | Phenolic. Quinone | 39.1 | 2678 |
phenaleno[2,3-g]quinolin-7-one | — | — | — | — | — | — | 2.88 | 7.94 | — | — | — | Alkaloid | 39.4 | 2702 |
2,2,3,3-TETRAFLUORO-5-(1,1,2,2-TETRAFLUOROETHOXY)-2,3-DIHYDROBENZOFURANOBENZOFURAN | — | 9.63 | 8.57 | — | — | — | 3.29 | 5.29 | 1.83 | 10.17 | 34.67 | Phenolic | 39.41 | 2703 |
Antra-9,10-quinone, 1-(3-hydrohy-3-phenyl-1-triazenyl)- | — | — | 2.86 | — | — | — | — | — | — | — | — | Phenolic. Quinone | 39.43 | 2704 |
6H-phenanthro[9,8-gh]quinolin-6-one | — | 2.96 | — | — | — | — | — | 20.11 | — | — | — | Alkaloid | 39.7 | 2728 |
2-Ethylacridine | — | — | 2.86 | — | — | — | — | — | — | — | — | Alkaloid | 39.82 | 2739 |
5-Methyl-2-phenylindolizine | — | 13.33 | — | — | — | — | — | — | — | — | — | Alkaloid | 40.4 | 2789 |
Cyclohexane-1,3-dione, 2-allylaminomethylene-5,5-dimethyl | — | — | — | — | 5.19 | — | — | — | — | — | — | Keton | 40.5 | 2797 |
4-Methoxy-3-(3-methoxyphenyl)-4-methylpentan-1-ol | — | — | — | — | — | — | — | — | 21.95 | — | — | Alcohol | 40.8 | — |
1,4-Phthalazinedione, 2,3-dihydro-6-nitro- | 2.54 | 54.81 | 19.05 | — | — | — | 32.1 | 11.11 | 28.05 | 11.86 | 19.77 | Luminole. Alkaloid | 41.37 | — |
demethoxy-12-epi-fumitremorgin B | — | 9.63 | — | — | — | — | 33.98 | — | — | 8.76 | — | Alkaloid | 41.4 | — |
7H-Dibenzo[b,g]carbazole, 7-methyl- | — | — | — | — | — | — | — | 19.58 | — | — | — | Alkaloid | 41.45 | — |
1H-Indole-2-carboxylic acid, 6-(4-ethoxyphenyl)-3-methyl-4-oxo-4,5,6,7-tetrahydro-, isopropyl ester xo-4,5,6,7-tetrahydro-, isopropyl ester | — | — | 52.38 | — | — | — | — | — | — | — | — | Alkaloid-indol | 41.47 | — |
Benzo[h]quinoline, 2,4-dimethyl- | — | 3.7 | — | — | 3.09 | — | — | 6.88 | 35.37 | — | — | Alkaloid | 41.6 | — |
2-(Acetoxymethyl)-3-(methoxycarbonyl)biphenylene | — | — | — | — | 37.68 | — | — | — | 8.54 | — | 27.51 | Aromatic | 43 | — |
Cyclopentanecarboxamide, 3-ethenyl-2-(3-pentenylidene)-N-phenyl-, [1.alpha.,2Z(E),3.alpha.]- | 41.53 | — | — | — | 20.44 | — | — | 17.46 | 2.44 | 27.4 | — | Aromatic ester | 43.55 | — |
Stigmastan-3,5-diene | 33.9 | — | — | — | — | — | — | — | — | — | — | Steroid | 44.3 | — |
Total identification | 84.75 | 95.54 | 97.15 | 95.75 | 75.45 | 92.01 | 80.6 | 95.77 | 98.18 | 82.74 | 96.85 | |||
Fatty acids | — | — | — | 4.41 | — | 12.19 | — | — | — | — | — | |||
Hydrocarbons-aliphatic | — | — | — | 70.51 | 4.45 | 79.82 | — | — | — | 7.91 | 5.73 | |||
Hydrocarbons-aromatic | 41.53 | — | — | — | 58.12 | — | — | 17.46 | 10.98 | 27.4 | 27.51 | |||
Operating agant hydrocarbon | 3.39 | — | — | 1.09 | 5.59 | — | — | — | 21.95 | — | — | |||
Alkaloids | 5.93 | 84.43 | 75.24 | — | 3.09 | — | 73.19 | 69.85 | 63.42 | 20.62 | 28.94 | |||
Phenolic compounds | — | 11.11 | 21.91 | — | — | — | 7.41 | 8.46 | 1.83 | 26.81 | 34.67 | |||
Sesquiterpenes | — | — | — | 1.9 | — | — | — | — | — | — | — | |||
Steroids | 33.9 | — | — | — | — | — | — | — | — | — | — | |||
Carboxylic acid | — | — | — | — | 4.2 | — | — | — | — | — | — | |||
Diterpenes | — | — | — | 7.2 | — | — | — | — | — | — | — | |||
