Figure 1

The concentration of soluble DNA-MPO complexes (a putative marker of in vivo NET generation/catabolism) was determine by ELISA (see methods) in platelet-free plasma samples of patients and of healthy controls (a) and in patients before and after gastric sleeve (b). Two different groups of subjects were observed among the patients studied: those that reduce (Group 1) (c) and those that increased (Group 2) (d) the fraction of plasmatic MPO-DNA complexes after sleeve gastrectomy. (e) The amount of MPO-DNA complexes observed in obese patients were compared to those observed in patients with history of acute myocardial infarction (AMI) or severe coronary artery disease but without previous AMI or unstable angina. In (a–d), each symbol depict the result of a single subject (patient or control). In (a,b), red lines indicates mean ± SD. In (e) ^§§P < 0.001; *P < 0.05; ^##P ≤ 0.001; °P < 0.05; P values were determined by Kruskal-Wallis and Mann-Whitney Tests.