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Figure 1

From: Molecular sexing and preliminary assessment of population sex ratio of the endangered Malayan tapir (Tapirus indicus) in Peninsular Malaysia

Figure 1

Amplification of the sex-identification region Y (SRY) and zinc finger (ZF) genes in 22 Malayan tapir individuals. (a) Male tapirs yielded amplicons of SRY at ~200 bp and ZFX/ZFY at ~450 bp. Female tapirs yielded only amplicons of ZFX at ~450 bp. ZF gene acts as a positive control for the PCR. (b) Sex-identification in 11 females and 11 males by multiplexing the primers of SRY and ZF genes in PCR, and the products were visualised on 2% agarose gel. All males and females show consistent sex-specific banding patterns. L – 50 bp ladder, N – negative control, M – male, F – female. Both images (a,b) were cropped from the full-length gels presented in Fig. S1 in Supplementary Information.

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