Figure 6 | Scientific Reports

Figure 6

From: Growth-related trophic changes of Thunnus thynnus as evidenced by stable nitrogen isotopic values in the first dorsal spine

Figure 6

The work scheme of laboratory analysis. (1) Collagen extraction: (a) extraction of first dorsal spine; (b) pieces of spine; (c,d) preparation to collagen extraction; (e) example of collagen extracted. (2) Analysis of whole spine: (a) spine; (b) steps to obtain powder of spine. (3) Powder of Opaque and Non-opaque bands: (a) section of the spine; (b) binocular to drilled the bands; (c) small drill; (d) powder obtained. (4) Weight in tin cups. (5) ThermoFisher Flash EA1112 elemental analyzer coupled to a Thermo Electron Delta Plus XP isotope ratio mass spectrometer (IRMS). (Thunnus thynnus picture permission granted by the author Andrea Paoli).

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