Figure 1
From: Microinjection induces changes in the transcriptome of bovine oocytes
Microinjection of bovine oocytes. Oocytes injected with non-specific siRNA mixed with Dextran-TRITC at the germinal vesicle stage and cultured in roscovitine for 16 h before maturation in vitro. (A) Oocytes at germinal vesicle (left), Metaphase I (middle) and Metaphase II (right) stages, based on chromatin distribution (DAPI staining in blue. Insets show magnified view of red encircled areas. Scale bar: 30 μm. (B) Bright field (left) and fluorescence (right) microscopic images of matured Metaphase II stage oocytes after siRNA injection. Red fluorescence indicates successful injection. Scale bars: 50 μm.
