Figure 5

Expansion of myeloid progenitors and CD34⁺Mac1⁺ myeloblasts in Mir142^–/– + IDH2^R140Q leukemias. (a) Proportion of GFP⁺ cells in the bone marrow from recipients of transduced HSPCs (WT + CTL n = 7, Mir142^–/– + CTL n = 5, WT + IDH2^R140Q n = 9, Mir142^–/– + IDH2^R140Q n = 10). (b) Proportion of Mac1⁺ cells within CD45.2⁺GFP⁺ population in peripheral blood from recipients of transduced HSPCs (WT + CTL n = 9, WT + IDH2^R140Q n = 12, Mir142 ^–/– + IDH2^R140Q n = 14). Arrow indicates an animal which had a lower proportion of Mac1 + cells due to the majority (66%) of CD45.2⁺GFP⁺ peripheral blood cells having a more primitive CD34⁺Mac1^– phenotype. (c) Percentage GFP⁺ cells in spleens from recipients of transduced HSPCs (WT + CTL n = 5, WT + IDH2^R140Q n = 7, Mir142 ^–/– + IDH2^R140Q n = 9). (d) Proportion of GFP⁺ cells in the livers from recipients of transduced HSPCs (WT + IDH2^R140Q n = 4, Mir142 ^–/– + IDH2^R140Q n = 4). (e) Spleen size in recipients of transduced HSPCs (WT + CTL n = 7, WT + IDH2^R140Q n = 11, Mir142 ^–/– + IDH2^R140Q n = 13). (f,g) Proportion of GMPs within GFP⁺ progenitors (c-Kit⁺ Lin^– Sca1^– compartment) in the bone marrow (f) or spleens (g) of recipients of transduced HSPCs (WT + IDH2^R140Q n = 6, Mir142^–/– + IDH2^R140Q n = 8). (h) Representative FACS plots showing proportion of CD34⁺ Mac1⁺ myeloblasts within the GFP⁺ population in the bone marrow (BM, top panels) or spleen (SP, lower panels) from recipients of transduced HSPCs. (i,j) Proportion of CD34⁺ Mac1⁺ myeloblasts within the GFP⁺ population in the bone marrow (i) or spleen (j) from recipients of transduced HSPCs (WT + IDH2^R140Q n = 6, Mir142^–/– + IDH2^R140Q n = 8). (k) Cytospin of Mir142^–/– + IDH2^R140Q CD34⁺Mac1⁺ cells showing myeloblastic morphology. Data in (a–c,e) is pooled from 3 to 4 independent experiments using both Mir142^em2Card (open circles) and Mir142^HOJ2 (filled circles) KO mice. Data in (f–j) generated using only the Mir142^em2Card allele, pooled from 2 independent experiments.