Figure 1 | Scientific Reports

Figure 1

From: Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock

Figure 1

Spatiotemporal expression profile of Gpr19 in the SCN. (A) Representative brain coronal sections of Gpr19+/+ and Gpr19−/− mice hybridised to anti-sense 33P-labelled Gpr19 riboprobe. Arrows indicate the position of the SCN. Scale bars, 1 mm. (B) RNAscope in situ hybridisation of Gpr19 in the SCN. The sections were counterstained with haematoxylin. Right panels show the extracts of the Gpr19-RNAscope signal. The dashed lines delineate the SCN. oc, optic chiasm; v, third ventricle. Scale bars, 50 μm. (C) Circadian rhythm of Gpr19 expression in the SCN. Relative mRNA abundance was determined by in situ hybridisation autoradiography. Values are presented as the mean ± SEM (n = 6, for each time point). Representative time-series autoradiographs are shown on top. Scale bars, 200 μm. (D) Western blots of Gpr19 in the SCN of Gpr19+/+ and Gpr19−/− mice at ZT4 and ZT16. Endogenous Gpr19 proteins were immunoprecipitated from hypothalamic SCN membrane extracts and probed for Gpr19. The solid and open arrowheads indicate Gpr19 and non-specific bands, respectively. Relative protein levels were determined by densitometry. P = 0.06, ZT4 vs. ZT16 in Gpr19+/+ SCN samples (n = 5), two-tailed Student's t-test. N.D., not detectable, in Gpr19−/− samples.

Back to article page