Table 2 Polymorphic markers used for linkage analysis.
From: QTL mapping for low temperature germination in rapeseed
Markers | Primer sequences | Chra | Locationb | |
|---|---|---|---|---|
Forward (5′-3′) | Reverse (5′-3′) | |||
Nys9A204 | GCATGTATAACTCCTTGAATCC | CCACAAGAAATTAGAGGTCG | A09 | 43716935–43717137 |
Nys9A207 | TACTCCTTTGGAAGGAAACA | TCCCTCTCCATCTGAAAATA | A09 | 44059061–44059257 |
Nys9A208 | ATTCTGTGTATCCCATTTCG | GAGGAGGTTTCTGAGGAGTT | A09 | 44230061–44230241 |
Nys9A145 | TTCAGTAAAGCTGACACACG | CGAGAGGTTATTAGGGGTTT | A09 | 44611834–44612080 |
Nys9A212 | CAAAAGAGGGAATTTCAGTG | TGTCTCTAGTGAGAAAGCATTG | A09 | 44721694–44721885 |
Nys9A215 | AACACACAGACATCGAGACA | TGAGATTGAAAGAGAAGGGA | A09 | 45063742–45063891 |
Nys9A216 | CCCTGGATATAGCTTGTGAT | AGACCTTTTTCATTGTCAGG | A09 | 45165658–45165884 |
Nys9A218 | TCTGGACAGCATCTTTAGGT | CTCAGACAACTCAGCAATCA | A09 | 45389536–45389768 |
Nys1C90 | ATAAATAGCCCCACGTCTCT | ACAGAGGAAATCGAAACAGA | C01 | 47748257–47748503 |
Nys1C91 | GGGAGGTTCTAGGACAAAAT | AGTGACAGATGGGATCAAAC | C01 | 47788771–47789026 |
Nys1C96 | GAGCTTAAGGGCTCTTCTTC | TAGGAGACTTGACCATCCAC | C01 | 48189600–48189816 |
Nys1C46 | CTGAAACCCAAAAATAGCG | TAGATCCAGCTAAGTACCCG | C01 | 48690912–48691125 |
Nys1C191 | CACGTACAATCAGTCAACCA | CCATCACCATGAAAATCTCT | C01 | 49158881–49159050 |
Nys1C117 | CTGGATAAAAAGAAACGTGG | TCTCTTCTTCATGCCTCTGT | C01 | 49503123–49503320 |
Nys1C137 | CTTTCTCCTCTTTTCCGATT | CCTTGCTTAGACTTCTTGGA | C01 | 51668433–51668607 |
Nys1C142 | TCCTTACCGCAAGAAACTAC | CGACGAGGAGTATGAGTAGG | C01 | 52183392–52183583 |
Nys1C76 | TTCTCTCTTGGGAAAAGTCA | AGCCTTTGAAGACTAAACCC | C01 | 54524191–54524377 |
