Figure 2
ROS accumulation was measured in cells overexpressing CaBir1, CaNma111, CaYbh3, CaDhh1, or CaPat1. The wild-type BWP17 and overexpression strains were grown to early log phase, incubated with 50 μg/ml H2DCFDA for 60 min, and then treated with 10 mM H2O2. ROS-stained cells were observed under an Olympus BX51 microscope with a 60 × objective. Graphs represent the quantification of ROS-stained cells (%) (n = 3 replicates, > 200 cells). Values are presented as the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.005 (compared with the wild-type BWP17).
