Table 3 The effects of bacterial inoculation, salinity levels and melatonin on nitrogen concentration and content, and content of protein in shoots and roots of common bean.

From: Co-application of ACC deaminase-producing rhizobial bacteria and melatonin improves salt tolerance in common bean (Phaseolus vulgaris L.) through ion homeostasis

Factor

Nitrogen concentration (%)

Nitrogen content (g pot−1)

Protein content (%)

Shoot

Root

Shoot

Root

Shoot

Root

Bacterial inoculations (I)

NI

1.42b

1.40b

14.14b

3.99b

8.87b

8.76b

RI

2.13a

1.69a

25.25a

5.58a

13.33a

10.60a

Salinity levels (S)

NS

2.08a

1.73a

26.63a

6.12a

13.02a

10.87a

S1

1.70b

1.57b

18.64b

4.79b

10.64b

9.85b

S2

1.54c

1.33c

13.83c

3.44c

9.63c

8.32c

Priming (P)

PM100

2.10a

1.68a

27.10a

6.02a

13.17a

10.52a

PH

1.56b

1.62a

17.55b

5.03b

9.75b

10.13a

PD

1.66b

1.34b

14.45c

3.30c

10.38b

8.39b

Bacterial inoculations × Salinity levels × Priming (I × S × P)

NI

NS

PM100

1.43g–j

1.75c

21.22de

7.04c

8.98g–j

10.94c

PH

1.82d–g

1.56de

19.60d–g

4.72e

11.39d–g

9.77de

PD

1.62f–i

1.38e–g

14.30h–j

3.48g–j

10.18f–i

8.66e–g

S1

PM100

1.21jk

1.26gh

12.60i–l

3.69f–i

7.56jk

7.90gh

PH

1.52g–j

1.54e

15.85f–i

4.50ef

9.54g–j

9.64e

PD

1.34ij

1.49ef

9.60kl

3.18h–j

8.38ij

9.35ef

S2

PM100

1.72e–i

1.12h

15.17g–j

2.84ij

10.80e–i

7.03h

PH

0.86k

1.34fg

8.20l

3.73f–h

5.37k

8.40fg

PD

1.22jk

1.14h

10.74j–l

2.69j

7.63jk

7.13h

RI

NS

PM100

3.42a

2.37a

54.80a

10.26a

21.42a

14.84a

PH

2.14b–d

2.00b

27.79c

7.17bc

13.39b–d

12.55b

PD

2.04c–e

1.35fg

22.05de

4.07e–g

12.77c–e

8.43fg

S1

PM100

2.52b

2.07b

35.25b

7.94b

15.75b

12.98b

PH

1.64f–i

1.72cd

20.37d–f

5.90d

10.28f–i

10.80cd

PD

1.97c–f

1.34fg

18.17e–h

3.52g–j

12.34c–f

8.42fg

S2

PM100

2.31bc

1.50ef

23.58cd

4.34e–g

14.49bc

9.43ef

PH

1.36h–j

1.53e

13.47i–k

4.16e–g

8.55h–j

9.60e

PD

Analysis of variance

I

***

***

***

***

***

***

S

***

***

***

***

***

***

P

***

***

***

***

***

***

I × S

ns

ns

***

***

ns

ns

I × P

***

***

***

***

***

***

S × P

***

***

***

***

***

***

I × S × P

***

***

***

*

***

***

  1. I, S and P are bacterial inoculations, salinity levels and priming treatments. NI and RI are non-inoculation and Rhizobium inoculated plants. NS, S1 and S2 are non-saline, 4 and 8 dSm−1 of salinity stress, respectively. PM100, PH and PD are melatonin priming, hydro priming and non-priming, respectively. ns, *, ** and *** indicate non-significant and significance at 5%, 1% and 0.1%, respectively. Different letters within a column indicate means that are significantly different at P < 0.05.