Figure 1 | Scientific Reports

Figure 1

From: HslO ameliorates arrested ΔrecA polA cell growth and reduces DNA damage and oxidative stress responses

Figure 1

Tolerance of polA25 cells for introducing a ΔrecA306 allele with P1 transduction. (a) Genome sequence reference mapping of AQ10459 and AQ10870 against the MG1655 genome and junction points. In (a), the top line indicates that the DNA region matches AQ10459 and AQ10870; left 2643 bp and right 1264 bp, or miss in AQ10870; centre 6849 bp with the gene location. Junction sequences of AQ10459 and AQ10870 are shown in the centre and bottom lines, respectively. The results of reference mapping are shown. (b) Analysis of recA alleles in ΔrecA polA candidate cells using long PCR. Long PCR was carried out as described in “Methods”. Results of 0.8% agarose gel electrophoresis are shown. Lane 1: marker, lane 2: AQ10459, lane 3: AQ10870, lane 4: ΔrecA306 derivative, lane 5: TK1230 cells, lane 6: TK1224 cells, lane 7: marker. Arrow heads indicate the long PCR product from recA+ (blue) or ΔrecA306 (red). The gel image in (b) shows a flipped image of the appropriate area without either the extra unnecessary markers or the gel origins from the original photograph (Fig. S2).

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