Table 2 Primer information.

From: Carvacrol attenuated lipopolysaccharide-induced intestinal injury by down-regulating TLRs gene expression and regulating the gut microbiota in rabbit

Gene

Accession no.

Forward primer (5′–3′)

Reverse primer (5′–3′)

ZO-1

100346390

GAGAACAAGAAGGAGGTGAA

CACTGAACTGGCTCTGAG

Occluding

100338492

TTGAGCAGCAGCAGTAAC

TGTAGTCCGTCTCGTAGTG

Claudin-1

NM_001089316

AATTCGGTCAGGCTCTTT

GAGGACAAGAACAGCAAAG

NF-κB

XM-008272930

GGCTCAACATCTACACAGT

GTCCTTGCGGAAGTCAAT

IL-1β

NM_001082201

GAATTTGAGTCTGCCCAGTT

CCATGCTGAAGTCAATTAGGT

IL-6

NM_001082064

CAAGTTCAGGAGTGACGAA

GAGGGTGGCTTCTTCATT

IL-8

NM_001082293

TGATGGAAGAGAACTCTGC

ATGACTCTTGCTGCTCAG

TNF-α

NM_001082263

AAGGTCAACCTCCTCTCT

CAGATAGATGGGCTCATACC

TLR2

NM_001082781.1

AGTACCAGAGGGTGTTACTG

GTAGCTCTTAGCACCTGTTC

TLR4

NM_001082732.2

AGATTGCTCAGACCTGGCAG

CACTGAGTCCAGAGGGAATG

MYD88

NW_003159756.1

GACTCCTGAGTGCCTCAGC

CTGCAGTCCCAGGGACCAG

MAPK14

XM_017345066.1

TGACCTGCTGGAGAAGATGC

CTGCTCATAGACGAGTGGAA

MAPK8

XM_002722670.3

GAGTGTGTTCTTACTTCATG

CCAAGTGTTGTGACTGTATC

MAPK1

XM_017349593.1

CCCTTCACAAGAAGACCTGA

CGAAGCTCCATTCAAGTTCG

GAPDH

NM_001082253.1

CTGAACGGGAAACTCACT

TCACCACCTTCTTGATGTC

  1. ZO-1: zonula occludens-1; IL-1β: interleukin 1 beta; IL-6: interleukin 6; IL-8: interleukin 8 beta; TNF-α: tumor necrosis factor alpha; TLR2: toll-like receptor 2; TLR4: toll-like receptor 4; MYD88: myeloiddifferentiationfactor 88; NF-κB: nuclear factor kappa-B; MAPK8: mitogen-activated protein kinase 8; MAPK14: mitogen-activated protein kinase 14; MAPK1: mitogen-activated protein kinase 1.
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