Figure 2 | Scientific Reports

Figure 2

From: Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-Rich (NCR) genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system

Figure 2The alternative text for this image may have been generated using AI.

The nodulation phenotype of hairy roots transformed with the construct pKSE466-RR-MtNCR169a was associated with the proportion of wild-type NCR169 allele or with the presence of mutant NCR169 lacking a single residue. White (Fix−) and pink (Fix+) nodules were randomly harvested 3–5 wpi with S. medicae WSM419 and the result of gene editing was identified by the sequencing of the targeted region using an Illumina platform. The ratios of reads assigned to mutant (brown ochre) and wild-type (blue) alleles are presented in pie charts. Fix- nodules (b,d, f) carried almost exclusively insertions or deletions resulting in frameshift in the coding sequence of NCR169 (a,c,e). Contrary, pink nodules (g,i,k) carried either unmodified alleles on a large scale (> 40%) (h,j) or a deletion of 3 base pairs that did not result in the loss of function of NCR169 (l).

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