Figure 5 | Scientific Reports

Figure 5

From: Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-Rich (NCR) genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system

Figure 5The alternative text for this image may have been generated using AI.

(a) Stable ncr068 mutants, regenerated from segments of transgenic hairy roots with induced mutations in NCR068 using the CRISPR method, retained wild type symbiotic phenotype similar to wild-type (WT) M. truncatula 2HA plants 3 wpi with S. medicae WSM419. (b,c) The gene edited nodules developed on A. rhizogenes-transformed hairy-roots were elongated and pink with colonized cells in the nitrogen fixation zone. (d,e) Nodules developed on the roots of regenerated stable ncr068 mutants were similar to the nodules found on hairy-roots mediated by A. rhizogenes. (f) The selected hairy root (NCR068-5_S138) was chimeric and composed of cells with homozygous insertions of A or G in the targeted region of NCR068. (g) The regenerated stable ncr068 mutants (Ac2211) carried a homozygous A insertion causing a frameshift in the coding sequence of NCR068. (h) The alignment of WT and mutant NCR068 peptide sequences encoded by the mutated gene in the stable ncr068 mutant. Identical residues are highlighted by black background, conserved Cys residues are in red. SP, signal peptide; MP, mature peptide.

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