Figure 6 | Scientific Reports

Figure 6

From: Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-Rich (NCR) genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system

Figure 6The alternative text for this image may have been generated using AI.

Stable ncr068 T1 mutant plants inoculated with three rhizobia strains show wild-type symbiotic phenotype. Wild-type M. truncatula 2HA (WT), the deletion mutant deficient in NCR169 (dnf7-2) and ncr068 mutant plants were inoculated with S. medicae WSM419, S. meliloti FSM-MA or S. meliloti 2011 and scored for symbiotic phenotype at 5 wpi. WT and ncr068 plants showed similar growth habit but presented less effective symbiotic interaction with S. meliloti 2011 compared with the two other strains. Mutant dnf7-2 showed nitrogen deficiency with all three tested rhizobia strains. SYTO13-staining revealed a similar colonization of WT and ncr068 nodules while only bacteria in infection threads and sporadic saprophytic bacteria fluoresced in the basal part of dnf7-2 nodules.

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