Table 3 Primer used for expression analysis of virulence genes and biofilm-forming genes in E.coli ATCC 25,922.
From: Evaluation of the application potential of Bdellovibrio sp. YBD-1 isolated from Yak faeces
Function | Genes | Primer sequence(5,-3,) | References |
|---|---|---|---|
Iron acquisition system | IroN | F: AAGTCAAAGCAGGGGTTGCCCG R: GACGCCGACATTAAGACGCAG | |
Adhesins | fim | F: GAGAAGAGGTTTGATTTAACTTATTG R: AGAGCCGCTGTAGAACTGAGG | |
pagA | F: TCTTGCGGCGTATATTGGTAGGT R: CGACCCGACAATCACCAGTACG | ||
Biofilm formation | pgaB | F: CGACGAAATGCGGCAATAACAC R: GCGGCGGCATATATTGTGGAAC | |
pgaC | F: TCACCATCGGGATCAGCAAAT R: GCAGCAGAATACCGGGAAAGA |
