Table 3 Gene annotation through in-silico analysis to identify candidate genes.

From: Discovering novel genomic regions explaining adaptation of bread wheat to conservation agriculture through GWAS

Trait

QTN

Candidate gene ID

Chromosome: location (bp)

Gene annotation

Phi2

QCALS.iari-1BL_Phi2

TraesCS1B02G343300

1B:571444691–571445480

Photosystem II reaction center W protein, chloroplastic

 

QCALS.iari-2DL_Phi2

TraesCS2D02G599200

2D:650684790–650686459

Cytochrome P450 89A9

NPQ

QCTTS.iari-5AL_NPQ

TraesCS5A02G314800

5A:525115605–525117944

UDP-glucose 4-epimerase 3

  

TraesCS5A02G314700

5A:525077777–525079517

Heat stress transcription factor B-2c

  

TraesCS5A02G314600

5A:525025721–525026755

Ethylene-responsive transcription factor ERF071

 

QCTLS.iari-4BS_NPQ

TraesCS4B02G116200

4B:133851917–133854945

UDP-glucose 6-dehydrogenase 4

 

QCTTS.iari-3DS_NPQ

TraesCS3D02G165300

3D:137002179–137006358

25.3 kDa vesicle transport protein

RC

QCTTS.iari-5AL_RC

TraesCS5A02G444500

5A:624706560–624708402

UDP-glycosyltransferase 73E1

 

QCTTS.iari-5BL_RC

TraesCS5B02G368900

5B:547652774–547657481

Chaperonin 60 subunit alpha 2, chloroplastic

 

QCTTS.iari-6BL_RC

TraesCS6B02G363300

6B:634501638–634502222

Putative ripening-related protein 5

 

QCTTS.iari-1DL_RC

TraesCS1D02G355200

1D:439878998–439883243

Hexokinase-2

  

TraesCS1D02G354100

1D:439414111–439415360

Peroxidase 1

 

QCTTS.iari-6DS_RC

TraesCS6D02G042400

6D:17309364–17310623

Putative wall-associated receptor kinase-like 16

  

TraesCS6D02G042300

6D:17297853–17304530

Wall-associated receptor kinase 2

CTD

QCTTS.iari-2BS_CTD

TraesCS2B02G111000

2B:72811061–72817327

Probable glutamyl endopeptidase, chloroplastic

  

TraesCS2B02G110800

2B:72608545–72619945

Lon protease homolog

PS1

QCATS.iari-2DL_PS1

TraesCS2D02G404800

2D:519132647–519135038

Aquaporin PIP1-2

  

TraesCS2D02G404600

2D:518969013–518971345

Cytochrome P450 86A22

  

TraesCS2D02G404500

2D:518810879–518814738

Thioredoxin-like 3–3

LT

QCALS.iari-4AL_LT

TraesCS4A02G192300

4A:472917832–472921560

Cyclin-dependent kinase B1-1

LA

QCATS.iari-1BS_LA

TraesCS1B02G004100

1B:2354630–2357674

Wall-associated receptor kinase 1

DTH

QCATS.iari-3AL_DTH

TraesCS3A02G422600

3A:663574417–663576507

Glycine-rich RNA-binding protein 4, mitochondrial

  

TraesCS3A02G421400

3A:662931519–662933524

Transcription factor bHLH49

  

TraesCS3A02G421900

3A:663258012–663265132

26S proteasome regulatory subunit 6A homolog A

 

QCTTS.iari-2BS_DTH

TraesCS2B02G015500

2B:7428456–7443833

Histone-lysine N-methyltransferase ASHH2

 

QCTTS.iari-4BLa_DTH

TraesCS4B02G374400

4B:658211040–658212993

GATA transcription factor 2

  

TraesCS4B02G373700

4B:657991716–657993829

VAN3-binding protein

  

TraesCS4B02G373600

4B:657989337–657991890

Formyltetrahydrofolate deformylase 2, mitochondrial

 

QCTTS.iari-4BLb_DTH

TraesCS4B02G398900

4B:673112846–673115678

Serine / arginine-rich splicing factor RS31

 

QCTTS.iari-6BL_DTH

TraesCS6B02G402700

6B:678744926–678745984

BTB / POZ and MATH domain-containing protein 1

 

QCTTS.iari-6BS_DTH

TraesCS6B02G110200

6B:92523435–92524395

18.9 kDa heat shock protein

  

TraesCS6B02G110400

6B:92697243–92698587

Probable xyloglucan endotransglucosylase/ hydrolase protein 30

  

TraesCS6B02G110600

6B:92749833–92755866

Ribose-phosphate pyrophosphokinase 1, chloroplastic

 

QCTTS.iari-7BL_DTH

TraesCS7B02G327700

7B:581892922–581894632

Probable xyloglucan endotransglucosylase/ hydrolase protein 23

  

TraesCS7B02G327600

7B:581794516–581796383

Inositol polyphosphate multikinase IPK2

  

TraesCS7B02G327400

7B:581372974–581376095

Xyloglucan endotransglucosylase/ hydrolase protein 22

 

QCATS.iari-1BL_DTH

TraesCS1B02G246600

1B:436536923–436537890

Protein SRC2

 

QCTLS.iari-1DL_DTH

TraesCS1D02G218700

1D:305586356–305590935

RNA-binding KH domain-containing protein PEPPER

DTM

QCTTS.iari-1AL_DTM

TraesCS1A02G433400

1A:584676043–584676930

Pentatricopeptide repeat-containing protein At2g15690, mitochondrial

 

QCATS.iari-3AL_DTM

TraesCS3A02G219300

3A:402135204–402136682

Protein tesmin / TSO1-like CXC 4

 

QCALS.iari-6BS_DTM

TraesCS6B02G028300

6B:16996335–16996970

MADS-box transcription factor PHERES 2

 

QCTTS.iari-1DL_DTM

TraesCS1D02G066600

1D:48021551–48024273

Importin subunit beta-1

  

TraesCS1D02G066400

1D:47934766–47936019

Polygalacturonase QRT2

 

QCTTS.iari-1DS_DTM

TraesCS1D02G136400

1D:181626866–181639553

Serine / threonine-protein kinase EDR1

 

QCATS.iari-2DS_DTM

TraesCS2D02G031400

2D:12619687–12623828

Transcription factor E2FB

  

TraesCS2D02G030800

2D:12550664–12552270

7-deoxyloganetin glucosyltransferase

PH

QCTTS.iari-2AS_PH

TraesCS2A02G041300

2A:16719074–16727514

Fatty acid amide hydrolase

  

TraesCS2A02G041500

2A:16745853–16746524

Ethylene-responsive transcription factor 1B

 

QCTTS.iari-5AL_PH

TraesCS5A02G548300

5A:702459427–702460136

PLASMODESMATA CALLOSE-BINDING PROTEIN 3

  

TraesCS5A02G548500

5A:702501770–702503426

Probable xyloglucan endotransglucosylase/ hydrolase protein 32

  

TraesCS5A02G548700

5A:702631484–702632670

Heat stress transcription factor C-2a

  

TraesCS5A02G549400

5A:703181022–703183341

UDP-glycosyltransferase 85A1

 

QCATS.iari-2DL_PH

TraesCS2D02G560400

2D:633258611–633259105

SKP1-like protein 1A

TC

QCTTS.iari-5AL_TC

TraesCS5A02G386600

5A:584415361–584420062

YTH domain-containing protein ECT2

 

QCTTS.iari-5BL_TC

TraesCS5B02G252600

5B:435597303–435598318

Probable E3 ubiquitin-protein ligase XERICO

  

TraesCS5B02G252500

5B:435156029–435159077

Serine / threonine-protein kinase D6PKL2

 

QCATS.iari-1DS_TC

TraesCS1D02G016800

1D:7583590–7587977

Wall-associated receptor kinase 2

SL

QCTLS.iari-2AS_SL

TraesCS2A02G212200

2A:197088104–197089901

Transcription factor UDT1

 

QCTTS.iari-6BL_SL,SPS

TraesCS6B02G360300

6B:632182793–632188473

Soluble inorganic pyrophosphatase 6, chloroplastic

  

TraesCS6B02G360500

6B:632304665–632305774

WAT1-related protein At2g39510

SPS

QCTTS.iari-6BL_SPS

TraesCS6B02G360300

6B:632182793–632188473

Soluble inorganic pyrophosphatase 6, chloroplastic

  

TraesCS6B02G360500

6B:632304665–632305774

WAT1-related protein At2g39510

GPS

QCTLS.iari-3BL_GPS

TraesCS3B02G303600

3B:487855328–487857841

Aldehyde oxidase GLOX1

  

TraesCS3B02G304300

3B:488542649–488550339

Protein MALE DISCOVERER 1

  

TraesCS3B02G304200

3B:488535427–488537668

Rop guanine nucleotide exchange factor 11

TGW

QCTTS.iari-3DS_TGW

TraesCS3D02G148000

3D:112694083–112698708

Sodium/calcium exchanger NCL1

  

TraesCS3D02G148200

3D:112960872–112963125

Basic leucine zipper 2

 

QCALS.iari-6DL_TGW

TraesCS6D02G362000

6D:452735812–452739910

SAP-like protein BP-73

GY

QCTTS.iari-5AL_GY

TraesCS5A02G500700

5A:666682422–666683774

NAC domain-containing protein 22

 

QCTTS.iari-3BS_GY

TraesCS3B02G052900

3B:26751728–26753832

Beta-1,2-xylosyltransferease XAX1

 

QCTTS.iari-7DL_GY

TraesCS7D02G516500

7D:616035255–616041322

Protein PSK SIMULATOR 1

  

TraesCS7D02G515100

7D:615774804–615776009

Transcription factor MYB44

GL

QCALS.iari-3AL_GL

TraesCS3A02G408200

3A:653303151–653306203

Bidirectional sugar transporter SWEET1a

  

TraesCS3A02G408000

3A:653240579–653244186

Scarecrow-like protein 1

GB

QCTTS.iari-5BL_GB

TraesCS5B02G241900

5B:421645424–421646515

Oil body-associated protein 1A

 

QCALS.iari-7DL_GB,GSA

TraesCS7D02G523600

7D:620116105–620117371

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3

GSA

QCALS.iari-1BL_GSA

TraesCS1B02G200300

1B:359198865–359209340

Tubulin-folding cofactor D

  1. Phi2 PS II quantum yield, NPQ non-photochemical quenching, RC relative chlorophyll, CTD canopy temperature depression, PS1 active PS I center, LT leaf thickness, LA leaf angle, DTH days to heading, DTM days to maturity, PH plant height, TC tiller count, SL spike length, SPS spikelets per spike, GPS grains per spike, TGW thousand-grain weight, GY grain yield GL grain length, GB grain breadth, GSA grain surface area.