Figure 1 | Scientific Reports

Figure 1

From: Vitamin D3 suppresses Npt2c abundance and differentially modulates phosphate and calcium homeostasis in Npt2a knockout mice

Figure 1The alternative text for this image may have been generated using AI.

Lack of Npt2a unravels a link of vitamin D3 on plasma Pi. Measurements of plasma and urinary Pi and Ca2+ were conducted in WT and Npt2a−/− mice after 4 days of treatment with either a vehicle or vitamin D3 (n = 6–10 per genotype). (a) In WT mice, plasma Pi levels remained unchanged following vitamin D3 treatment. (B) In contrast, lower plasma Pi levels under baseline conditions in Npt2a−/− mice significantly increased in response to vitamin D3 treatment. (c) The urinary Pi/creatinine ratio in WT mice increased significantly in response to vitamin D3 treatment. (d) This ratio in Npt2a−/− mice was unchanged (d). Plasma Ca2+ levels in both WT and Npt2a−/− mice showed a significant increase following vitamin D3 treatment (e & f). In WT mice, the urinary Ca2+ to creatinine ratio significantly increased after vitamin D3 treatment (g). In contrast, this ratio significantly decreased in Npt2a−/− mice (h). Male mice were used in these studies. In addition to single data summary data are shown and are expressed as mean ± SEM and were analyzed by repeated-measures two-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05 vs WT same time point, #P < 0.05 vs baseline same genotype, §P < 0.05 vs vehicle same genotype and time point.

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