Figure 3 | Scientific Reports

Figure 3

From: Vitamin D3 suppresses Npt2c abundance and differentially modulates phosphate and calcium homeostasis in Npt2a knockout mice

Figure 3The alternative text for this image may have been generated using AI.

Npt2a determines the effects of Vitamin D3 on bone remodeling markers. Circulating bone markers, including osteocalcin, PINP, TRAcP 5b, and CTX-1 were measured in WT and Npt2a−/− mice after 4 days of treatment with either a vehicle or vitamin D3 (n = 6 per genotype). (a & b) Both genotypes show a small but significant increase in osteocalcin levels independent of treatment. (c & d) Vitamin D3 decreased plasma PINP independent of genotype. (e & f) In both genotypes, vehicle treatment slightly but significantly decreased plasma TRAcP 5b levels but vitamin D3 only significantly increased TRAcP 5b in WT mice. (g & h) Vitamin D3 treatment increased CTX-1 levels in both genotypes but to a significantly greater extent in Npt2a−/− mice. Male mice were used in these studies. In addition to single data summary data are shown and are expressed as mean ± SEM and were analyzed by repeated-measures two-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05 vs WT same time point, #P < 0.05 vs baseline same genotype, §P < 0.05 vs vehicle same genotype and time point.

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