Figure 4

Similar effects of acute oral Pi loading on plasma Pi levels between genotypes. Plasma Pi levels were measured in WT and Npt2a−/− mice before and one hour after oral Pi loading via gavage (0.5 mol*L−1, 1% of body weight). These measurements were performed following 4 days of treatment with either a vehicle or vitamin D3 (n = 6 per genotype). (a & b) Oral Pi loading significantly increased plasma Pi levels independent of genotype or treatment; however, in vitamin D3-treated mice the increase was significantly greater compared to vehicle-treated mice. Male mice were used in these studies. In addition to single data summary data are shown and are expressed as mean ± SEM and were analyzed by repeated-measures two-way ANOVA followed by Tukey’s multiple comparisons test. *P < 0.05 vs WT same time point, #P < 0.05 vs baseline same genotype, §P < 0.05 vs vehicle same genotype and time point.