Figure 1 | Scientific Reports

Figure 1

From: Deciphering the role of protein kinase A in the control of FoxP3 expression in regulatory T cells in health and autoimmunity

Figure 1

Low TCR stimulation activates PKA in human Tconv cells. (a) Scatter plots showing PKA activity in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated for 5 and 15 min. Data are shown from four independent experiments in duplicates (n = 8). (b) Left, immunoblot showing phosphorylated (p) and total CREB in human mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 15 min. Right, relative densitometric quantitation of p-CREB in the aforementioned experimental conditions. Data are shown from nine independent experiments (n = 9); uncropped blots are presented in Supplementary Fig. S2. (c) ChIP assay for CREB on FoxP3 promoter, CNS2 and CNS3 regions of mPKAI-Tconv and CTR-Tconv cells TCR-stimulated for 10 min. The horizontal line (bracket ± SEM) indicates the percent of input from a control ChIP (Ab:non-immune serum). Data are shown from three independent experiments in duplicates (n = 6). Independet experiments refer to different individuals.

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