Figure 2 | Scientific Reports

Figure 2

From: Deciphering the role of protein kinase A in the control of FoxP3 expression in regulatory T cells in health and autoimmunity

Figure 2

Inhibition of PKA impairs FoxP3 induction. (a) Scatter plots showing FoxP3-All (left) and FoxP3-E2 (right) mRNA levels in mPKAI-Tconv and CTR-Tconv cells TCR-stimulated or not for 24 or 36 h, respectively. Data are shown from four independent experiments in duplicates (n = 8). (b) Left, immunoblot analysis of FoxP3-All, FoxP3-E2 and ERK1/2 in mPKAI-Tconv and CTR-Tconv cells TCR-stimulated for 36 h. Right, relative densitometric quantitation of FoxP3-All or FoxP3-E2 normalized on ERK 1/2 in the aforementioned experimental conditions. Data are shown from thirteen independent experiments (n = 13). (c) Left, immunoblot analysis of p-STAT5 and p-S6 in human Tconv cells, as described in B. Right, relative densitometric analysis of p-STAT5 and p-S6 normalized on their total proteins, respectively. Data are shown from five independent experiments in duplicates (n = 10). Independet experiments refer to different individuals. All the uncropped blots are presented in Supplementary Fig. S3.

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