Figure 5
From: Attenuation of esophageal anastomotic stricture through remote ischemic conditioning in a rat model
Real-time quantitative polymerase chain reaction and western blot analysis of factors associated with IL-6. (A) Real-time quantitative polymerase chain reaction analysis of IL-6 in the rat esophageal tissue from the control, R&A-only, RIC1, and RIC2 groups (n = 10). The results are normalized to values obtained for the control group (value = 1). (B) Western blot analysis of p-JAK, JAK, p-STAT3, STAT3, and IL-6 in esophageal tissue samples from each group (n = 10). The relative band intensity is presented. (C, D) Quantification of western blot data from (B) using quantification software (Image J, Version 1.54j, https://imagej.net/ij), represented as the relative values of p-JAK compared with JAK and p-STAT3 compared with STAT3. All Data are means ± SEM p‐value was calculated by two‐way ANOVA with Tukey’s multiple comparison tests and also presented compared with the R&A-only group (n = 10) (ns = not significant, *p < 0.05, **p < 0.001, ***p < 0.0001). Abbreviations: R&A resection and anastomosis; RIC1 remote ischemic conditioning performed once postoperatively; RIC2 remote ischemic conditioning performed twice postoperatively; SEM standard error of the mean; JAK Janus kinase; p-JAK phosphorylated JAK; p-STAT3 phosphorylated STAT3; IL-6 interleukin-6.
