Table 1 Details of primers designed and ‘azenta’ IDs.
Sr. No | Gene names | Primer sequence/ID | GC% | Tm | BP | Size (bp) |
|---|---|---|---|---|---|---|
1 | TP53-F | 5ʹTTCGACATAGTGTGGTGGTG 3ʹ ID: S2052409355K | 50 | 57.9 | 20 | 487 |
TP53-R | 5ʹCCCTTTTTGGACTTCAGGTG 3ʹ ID: S2052409356K | 50 | 59.5 | 20 | ||
2 | BcL2-F | 5ʹGGGATTCCTGCGGATTGACA 3ʹ ID: S2052409351K | 55 | 60 | 20 | 551 |
BcL2-R | 5ʹTCCCGGTTATCGTACCCTGT 3ʹ ID: S2052409352K | 55 | 60 | 20 | ||
3 | Hprt1-F | 5ʹCGAACCTCTCGGCTTTCC 3ʹ ID: S2052409353K | 60 | 60.8 | 18 | 455 |
Hprt1-R | 5ʹTCCCCTGTTGACTGGTCATT 3ʹ ID: S2052409354K | 50 | 60.3 | 20 |
