Table 2 Optimized thermal profile for qPCR.

From: GC–MS analysis, molecular docking, and apoptotic-based cytotoxic effect of Caladium lindenii Madison extracts toward the HeLa cervical cancer cell line

qPCR steps

Temperature

Duration

Repeat

Pre-denaturation

94

10 min

1

Denaturation

94

15 s

35

Annealing

60

15 s

Elongation

72

15 s

Final elongation

72

10 min

1

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