Table 5 Gene expression analysis of TP53, Bcl-2, and HPRT1 genes by real-time PCR.
Ct | Min | Max | Range | SD | Mean | Ct | Min | Max | Range | SD | Mean | ΔCt | ΔΔCt M-Value | Fold-change | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TP53 | HPRT1 | ||||||||||||||
CAL-HeLa-T | 15.85 | 15.85 | 16.82 | 0.97 | 0.42 | 16.44 | 25 | 25.00 | 25.00 | 0.00 | 0.00 | 25.00 | − 8.56 | − 4.16 | 0.06 |
16.64 | 25 | ||||||||||||||
16.82 | 25 | ||||||||||||||
CAL-HeLa-C | 22.75 | 19.41 | 22.75 | 3.34 | 1.48 | 20.67 | 25 | 25.00 | 25.20 | 0.20 | 0.09 | 25.07 | − 4.40 | ||
19.41 | 25 | ||||||||||||||
19.84 | 25.2 | ||||||||||||||
BCL2 | HPRT1 | ||||||||||||||
CAL-HeLa-T | 20.3 | 18.88 | 20.30 | 1.42 | 0.60 | 19.70 | 25 | 25.00 | 25.00 | 0.00 | 0.00 | 25.00 | − 5.30 | 3.23 | 9.40 |
19.91 | 25 | ||||||||||||||
18.88 | 25 | ||||||||||||||
CAL-HeLa-C | 16.32 | 15.85 | 17.22 | 1.37 | 0.57 | 16.46 | 25 | 25.00 | 25.00 | 0.00 | 0.00 | 25.00 | − 8.54 | ||
15.85 | 25 | ||||||||||||||
17.22 | 25 | ||||||||||||||
