Figure 1

Feeding behaviour of female mosquitoes raised on blood versus mosquitoes raised on BLOODless diet. (A) Appetite and attraction to a standard vertebrate bloodmeal of female mosquitoes raised on blood versus mosquitoes raised on BLOODless diet. Three independent experiments were performed for each generation and colony. The feeding proportion values are represented as the mean + SD of three feeding experiments. Significantly different proportion of feeding was detected between Blood and BLOODless diet, controlling for generation (ANCOVA F1,21 = 12.099, P = 0.002); (B) Dietary choice between a standard vertebrate bloodmeal or a blood-free diet. Three independent experiments were performed on the 38th generation of female mosquitoes originating from both insectaries (Blood or BLOODless). Results are expressed as a percentage of the feeding rate for each type of meal. In grey are represented the female mosquitoes that did not feed. On the left side of the plot are the results for females originating from the BLOODless diet whereas on the right side of the plot are represented the results for female mosquitoes from the Blood colony. No significant differences were found between insectaries regarding the proportions of females preferring Blood (Mann–Whitney, p = 0.7).