Table 1 TNF-a, IL-6, IL-1β and GAPDH oligonucleotide primers were as follows.
From: Tetramethylpyrazine ameliorates LPS-induced acute lung injury via the miR-369-3p/DSTN axis
Genes (mouse) | Forward primer (5′–3′) | Reverse primer (3′–5′) |
|---|---|---|
TNF-α | CAGGCGGTGCCTATGTCTC | CGATCACCCCGAAGTTCAGTAG |
IL-6 | CTGCAAGAGACTTCCATCCAG | AGTGGTATAGACAGGTCTGTTGG |
IL-1β | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
GAPDH | TGACCTCAACTACATGGTCTACA | CTTCCCATTCTCGGCCTTG |
