Table 2 Effects of hormone administration method on fetal development of oocytes in xenotransplanted ovarian follicles from SD rats.

From: Generation of rat offspring from ovarian oocytes by xenotransplantation

Hormone admin

Embryo stage transferred

Number of

Implantation scar

Embryos

Recipients

Fetus

Pups

Found

Not found

Unconfirmed

PMSG & hCG*

Pronucleus

73

6

1

0

0

0

5

2 cell

36

2

0

0

0

0

2

PMSG × 2**

Pronucleus

95

6

0

0

2

3

1

2 cell

22

3

1

0

0

2

0

PMSG × 2***

Pronucleus

73

3

0

1

0

1

1

2 cell

89

7

0

0

0

2

5

  1. *48 h after PMSG (5 IU), hCG (5 IU) was administered, and 5 h later oocytes were retrieved.
  2. **48 h after PMSG (5 IU), PMSG (5 IU) was again given and 21 h later oocytes were retrieved.
  3. ***48 h after PMSG (5 IU), PMSG (5 IU) was again given and 29 h later oocytes were retrieved. Obtained fertilized eggs were divided into two groups, one in a pronuclear stage at 5–6 h after IVF and the other in a 2-cell stage at 24 h after IVF.
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