Fig. 6
The histological and immunohistochemical findings. (A) Grading for adipocytes on H&E staining of the M-T region (x200). Black arrows are adipocytes. (B) S100 stained cells in the M-T region (x400). Black arrows are S100-stained adipose cells. The G3 and G4 groups showed fewer adipocytes than the G2 groups. (C) CD 68-stained cells in the M-T region (x400). Black arrows are CD 68-stained cells. The G3 and G4 groups showed fewer CD68-stained cells than the G2 group. The G3 group showed the lowest number of CD 68-stained cells. (D) CD 168-stained cells in the M-T region (x400). Black arrows are CD168-stained cells. The G3 and G4 groups showed more CD168-stained cells than the G1 and G2 groups. (E) Collagen fiber continuity and parallel orientation on Masson’s trichrome stain (x100) of T-B junction. Black arrows are T-B junctions. The G3 and G4 groups showed more continuous and parallelly oriented collagen fibers than the G2 group. The G1 and G2 groups showed no definite T-B junctions. (F) CSA on H&E staining of the M-T region (x400). The G3 group showed the largest CSA, and the G2 group showed the smallest CSA. 1: sham 1; 2: sham 2; 3: G1; 4: G2; 5: G3; 6: G4. Sham 1 and 2: left shoulder of G1 and G2. G1: Group 1; saline + repair; G2: Group 2, saline + repair; G3: Group 3, PDRN + repair; G4: Group 4, PN + repair. G1: normal, G2-4: diabetes mellitus, M-T: musculotendinous, T-B; tendon to bone, CSA: cross-sectional area of the muscle fiber.
