Fig. 1
From: Two Tetrahymena kinesin-9 family members exhibit slow plus-end-directed motility in vitro
Tetrahymena kinesin-9 constructs used in this study. (a) Phylogenetic tree constructed with full-length kinesin-9 family amino acid sequences using maximum likelihood phylogenetic analyses, and 500 bootstrap resampling values (on nodes). Kinesin-9 can be divided into two subfamilies: kinesin-9A and kinesin-9B. The scale shows the estimated branch length, which represents the number of changes per site. (b) Coiled-coil (CC) probability plots for TtK9A (red) and TtK9B1 (blue) generated by Marcoil. (c) Schematic diagram of wild-type Tetrahymena kinesin-9 and the truncated kinesin-9 constructs used in this study. All constructs have a biotinylated peptide and affinity tag (AviHis). Values in brackets are theoretical molecular weights. (d) SDS-PAGE analysis of purified proteins (asterisks): 9A, 9A-GFP, 9A-SG, 9B1, and 9B1-GFP represent TtK9A_473, TtK9A_473-mEGFP, TtK9A_473-mStayGold, TtK9B1_506, and TtK9B1_506-mEGFP, respectively.
