Fig. 2
From: Two Tetrahymena kinesin-9 family members exhibit slow plus-end-directed motility in vitro
Microtubule gliding assays driven by Tetrahymena kinesin-9 molecules. (a) Schematic diagram of the in vitro microtubule gliding assay. Biotinylated kinesins are anchored to the streptavidin-coated glass surface. (b, c) Kymographs of microtubule gliding driven by TtK9A_473 and TtK9B1_506. (d, e) Histograms of microtubule gliding velocities for TtK9A_473 (n = 114) and TtK9B1_506 (n = 160). Each histogram is fitted to a Gaussian distribution; mean ± SD. Only gliding microtubules that were > 2 μm in length, moved > 2 µm, and did not cross each other were analyzed.
