Table 1 The primers used for RT-qPCR detection.
From: Metformin enhances osteogenic differentiation of BMSC by modulating macrophage M2 polarization
Gene | Forward primer sequences (5′-3′) | Reverse primer sequences (5′-3′) |
|---|---|---|
Arg-1 | AGCTCTGGGAATCTGCATGG | ATGTACACGATGTCTTTGGCAGATA |
CD206 | ACAAAGGGACGTTTCGGTG | TGGACATTTGGGTTCAGGAG |
IL-10 | GCCAGAGCCACATGCTCCTA | GATAAGGCTTGGCAACCCAAGTAA |
iNOS | TTTGCTTCCATGCTAATGCGAAAG | GCTCTGTTGAGGTCTAAAGGCTCCG |
IL-1β | AATCTATACCTGTCCTGTGTAATGA AAGAC | TGGGTATTGCTTGGGATCCA |
IL-6 | CTTGGGACTGATGCTGGTGACA | GCCTCCGACTTGTGAAGTGGTA |
TNF-α | GGAGGGGTCTTCCAGCTGGAGA | CAATGATCCCAAAGTAGACCTGC |
Runx2 | AACTTGCTAACGTGAATGGTC | TAGCCCACTGAAGAAACTTGG |
ALP | ACCACCACGAGAGTGAACCA | CGTTGTCTGAGTACCAGTCCC |
OCN | GAACAGACAAGTCCCACACAG | TCAGCAGAGTGAGCAGAAAGAT |
COL-1 | GAGTCAGCAGATTGAGAACATCC | AGTCAGAGTGGCACATCTTGAG |
GADPH | AGGTCGGTGTGAACGGATTTG | GGGGTCGTTGATGGCAACA |
